Hotspot

trnV-GAC-rrn16S

IRb · 105209-105430 · 222 bp

Back to Styphnolobium

Pi 0.0225

MarkerSeek score 42.60

Primer available yes

Label trnV-GAC-rrn16S

Feature Metadata

label_name: trnV-GAC-rrn16S
feature_type: igs
region: IRb
length_bp: 222
variable_sites: 10
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16S_p1	AAGGGGTATAACTCAGCGGT	CCAGGATCGAACTCTCCATG	50.0	55.0	58.1	57.5	318 bp	1.0	77.3
trnV-GAC-rrn16S_p2	TTGACGTGGTGGAAGTCATC	CCAGGATCGAACTCTCCATG	50.0	55.0	57.8	57.5	287 bp	1.0	77.3
trnV-GAC-rrn16S_p3	CGTGGTGGAAGTCATCAGTT	CCAGGATCGAACTCTCCATG	50.0	55.0	57.8	57.5	283 bp	1.0	77.3
trnV-GAC-rrn16S_p4	CTTGACGTGGTGGAAGTCAT	CCAGGATCGAACTCTCCATG	50.0	55.0	57.8	57.5	288 bp	1.0	77.2
trnV-GAC-rrn16S_p5	TCTATTTCTCGATGGGGGCA	CCAGGATCGAACTCTCCATG	50.0	55.0	58.2	57.5	342 bp	1.0	76.6