Hotspot

rps11-rpl36

LSC · 83601-84112 · 512 bp

Back to Styphnolobium

Pi 0.000977
MarkerSeek score 32.80
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
512
variable_sites
1
pi
0.000977
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 ATTTTACGCGCATGCTTACG CGGGGGCGAATTATAGTAAT 45.0 45.0 57.9 54.8 643 bp 1.0 53.7
rps11-rpl36_p2 ATTTTACGCGCATGCTTACG CGGGGGCGAATTATAGTAATTTG 45.0 43.5 57.9 57.7 643 bp 1.0 53.1
rps11-rpl36_p3 TTGGTATTTTACGCGCATGC CGGGGGCGAATTATAGTAAT 45.0 45.0 57.8 54.8 648 bp 1.0 52.9
rps11-rpl36_p4 CCCTTTGGTATTTTACGCGC CGGGGGCGAATTATAGTAAT 50.0 45.0 57.8 54.8 652 bp 1.0 52.9
rps11-rpl36_p5 TTGGTATTTTACGCGCATGC CGGGGGCGAATTATAGTAATTTG 45.0 43.5 57.8 57.7 648 bp 1.0 52.3