Hotspot

trnP-GGG-psaJ

LSC · 70328-71070 · 743 bp

Back to Styphnolobium

Pi 0

MarkerSeek score 49.40

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	838-861 bp	1.0	80.6
trnP-GGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	837-860 bp	1.0	80.6
trnP-GGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	849-872 bp	1.0	80.5
trnP-GGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	848-871 bp	1.0	80.5
trnP-GGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	50.0	50.0	58.1	57.8	851-874 bp	1.0	80.5