Hotspot

rbcL-accD

LSC · 59530-60500 · 971 bp

Back to Styphnolobium

Pi 0.000515

MarkerSeek score 51.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 1
pi: 0.000515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	CGGGTCTTCACTTACACTGG	50.0	55.0	58.0	57.9	1155-1170 bp	1.0	80.3
rbcL-accD_p2	CTGCCAAATGGAGTCCTGAA	CGGGTCTTCACTTACACTGG	50.0	55.0	57.8	57.9	1166-1181 bp	1.0	78.9
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	CGGGTCTTCACTTACACTGG	50.0	55.0	57.5	57.9	1143-1158 bp	1.0	76.3
rbcL-accD_p4	AGTCCTGAATTAGCTGCTGC	ACCCACGCCTGTATTCTAAC	50.0	50.0	58.0	57.3	1096-1111 bp	1.0	75.5
rbcL-accD_p5	CTTGCTCGTGAGGGTAATGA	CGGGTCTTCACTTACACTGG	50.0	55.0	57.3	57.9	1200-1215 bp	1.0	75.2