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atpH-atpI

LSC · 14274-15647 · 1374 bp

Back to Styphnolobium

Pi 0.000364

MarkerSeek score 46.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1374
variable_sites: 1
pi: 0.000364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.0	57.2	1465-1485 bp	1.0	74.8
atpH-atpI_p2	GATCCCTTCTACAGCTTGCC	ACAAGCGGTATTCAAGCTCT	55.0	45.0	58.0	57.2	1550-1570 bp	1.0	74.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.2	57.2	1466-1486 bp	1.0	73.6
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAAGGC	45.0	50.0	58.0	57.0	1416-1436 bp	1.0	72.7
atpH-atpI_p5	GATCCCTTCTACAGCTTGCC	TAGGTGAATCCATGGAAGGC	55.0	50.0	58.0	57.0	1501-1521 bp	1.0	72.7