Hotspot

rpl32-trnL-UAG

SSC · 115189-115961 · 773 bp

Back to Stylosanthes

Pi 0.0393

MarkerSeek score 77.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 773
variable_sites: 55
pi: 0.0393
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGAAACGCACCTCGAGATC	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1544-1604 bp	1.0	85.5
rpl32-trnL-UAG_p2	AAGAAACGCACCTCGAGATC	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1543-1603 bp	1.0	85.5
rpl32-trnL-UAG_p3	GGAGAGGTTTTTGGACAGCA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1492-1552 bp	1.0	85.5
rpl32-trnL-UAG_p4	GGAGAGGTTTTTGGACAGCA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1491-1551 bp	1.0	85.5
rpl32-trnL-UAG_p5	AAGAAACGCACCTCGAGATC	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1428-1488 bp	1.0	85.3