Hotspot

rps3-rps19

LSC · 85497-86025 · 529 bp

Back to Stylosanthes

Pi 0.0382
MarkerSeek score 61.70
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
529
variable_sites
37
pi
0.0382
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCGGAAACCAAGC TGATAATAGGTCTCGTCGTT 50.0 40.0 58.1 53.3 591-592 bp 1.0 47.5
rps3-rps19_p2 TCGGAAACCAAGCGGATTTA TGATAATAGGTCTCGTCGTT 45.0 40.0 57.5 53.3 584-585 bp 1.0 45.8
rps3-rps19_p3 TACCAAGTCGGAAACCAAGC ATGATAATAGGTCTCGTCGT 50.0 40.0 58.1 53.1 592-593 bp 1.0 45.7
rps3-rps19_p4 TACCAAGTCGGAAACCAAGC AATGATAATAGGTCTCGTCGT 50.0 38.1 58.1 54.0 593-594 bp 1.0 45.7
rps3-rps19_p5 TACCAAGTCGGAAACCAAGC ATGATAATAGGTCTCGTCGTT 50.0 38.1 58.1 54.0 592-593 bp 1.0 45.7