Hotspot

rps11-rpl36

LSC · 80776-81351 · 576 bp

Back to Stylosanthes

Pi 0.0316

MarkerSeek score 69.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 576
variable_sites: 34
pi: 0.0316
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	GATTAGAACGAATCGGCGGA	50.0	50.0	58.1	57.9	1237-1244 bp	1.0	82.6
rps11-rpl36_p2	TGAATCCACAAGTACCTGCG	GATTAGAACGAATCGGCGGA	50.0	50.0	57.9	57.9	1377-1384 bp	1.0	82.5
rps11-rpl36_p3	TGAATCCACAAGTACCTGCG	ATTAGAACGAATCGGCGGAG	50.0	50.0	57.9	57.9	1376-1383 bp	1.0	82.5
rps11-rpl36_p4	AGCTCTTAATGCCGCATCTC	GATTAGAACGAATCGGCGGA	50.0	50.0	58.1	57.9	1517-1524 bp	1.0	82.0
rps11-rpl36_p5	GCTCTTAATGCCGCATCTCT	GATTAGAACGAATCGGCGGA	50.0	50.0	58.1	57.9	1516-1523 bp	1.0	82.0