Hotspot

trnP-UGG-psaJ

LSC · 67712-68125 · 414 bp

Back to Stylosanthes

Pi 0.025

MarkerSeek score 64.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 19
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	516-525 bp	1.0	80.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	515-524 bp	1.0	80.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	527-536 bp	1.0	80.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	526-535 bp	1.0	80.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	529-538 bp	1.0	80.4