Hotspot

trnW-CCA-trnP-UGG

LSC · 67429-67637 · 209 bp

Back to Stylosanthes

Pi 0.0458

MarkerSeek score 79.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 17
pi: 0.0458
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	256-265 bp	1.0	86.7
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	254-263 bp	1.0	86.7
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	257-266 bp	1.0	86.6
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	267-276 bp	1.0	86.4
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	268-277 bp	1.0	86.4