Hotspot

petA-psbJ

LSC · 63670-64726 · 1057 bp

Back to Stylosanthes

Pi 0.0253

MarkerSeek score 66.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 49
pi: 0.0253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCTGCTACTGAAAGCGATGT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2096 bp	0.25	49.8
petA-psbJ_p2	CCTGCTACTGAAAGCGATGT	ACGATGTTTTTGGAAGCCCT	50.0	45.0	58.0	58.0	2163 bp	0.25	49.8
petA-psbJ_p3	CCTGCTACTGAAAGCGATGT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2095 bp	0.25	49.8
petA-psbJ_p4	CCTGCTACTGAAAGCGATGT	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2198 bp	0.25	49.7
petA-psbJ_p5	CCTGCTACTGAAAGCGATGT	TTAGGAGGCTCCAATGACCA	50.0	50.0	58.0	58.0	2045 bp	0.25	49.6