Hotspot

atpA-trnR-UCU

LSC · 53400-53683 · 284 bp

Back to Stylosanthes

Pi 0.0298

MarkerSeek score 51.40

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 15
pi: 0.0298
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CTCCAGGAACAAGTAGAAAA	GGTTCAAATCCTATTGGACGC	40.0	47.6	52.4	57.3	330-343 bp	1.0	44.5
atpA-trnR-UCU_p2	CTCCAGGAACAAGTAGAAAAA	GGTTCAAATCCTATTGGACGC	38.1	47.6	53.4	57.3	330-343 bp	1.0	44.5
atpA-trnR-UCU_p3	CTCCAGGAACAAGTAGAAAA	GTTCAAATCCTATTGGACGCA	40.0	42.9	52.4	56.6	329-342 bp	1.0	44.5
atpA-trnR-UCU_p4	CTCCAGGAACAAGTAGAAAA	TGGATAGGACAGAGGTCTTCT	40.0	47.6	52.4	56.9	365-378 bp	1.0	44.1
atpA-trnR-UCU_p5	CTCCAGGAACAAGTAGAAAAA	TGGATAGGACAGAGGTCTTCT	38.1	47.6	53.4	56.9	365-378 bp	1.0	44.1