Hotspot

trnT-GGU-trnE-UUC

LSC · 31072-31879 · 808 bp

Back to Stylosanthes

Pi 0.0274

MarkerSeek score 71.00

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 40
pi: 0.0274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	AGAGAGATGTCCTGGACCAC	50.0	55.0	58.0	58.1	888-916 bp	1.0	84.0
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	GAGAGATGTCCTGGACCACT	50.0	55.0	58.0	58.1	887-915 bp	1.0	84.0
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.0	57.8	903-931 bp	1.0	83.7
trnT-GGU-trnE-UUC_p4	CCATGGCGTTACTCTACCAC	GAGAGATGTCCTGGACCACT	55.0	55.0	58.1	58.1	866-894 bp	1.0	83.7
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	940-968 bp	1.0	83.6