Hotspot

trnG-UCC-psbZ

LSC · 25313-26151 · 839 bp

Back to Stylosanthes

Pi 0.0366

MarkerSeek score 74.80

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 42
pi: 0.0366
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CCGGTACCTCATTATGGATTGT	50.0	45.5	57.8	57.6	835-951 bp	1.0	67.6
trnG-UCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	GATGGTTGGTCGAGTAACAA	50.0	45.0	57.8	55.4	869-985 bp	1.0	65.6
trnG-UCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCCGGTACCTCATTATGGATTG	50.0	45.5	57.8	57.4	836-952 bp	1.0	65.5
trnG-UCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	TGTTGTATTTTCCGGTACCTCA	50.0	40.9	57.8	57.4	846-962 bp	1.0	65.4
trnG-UCC-psbZ_p5	AAAGTAATAAGGGCAGGGGC	CCGGTACCTCATTATGGATTGT	50.0	45.5	57.5	57.6	992 bp	0.25	27.6