Hotspot

trnV-UAC-ndhC

LSC · 10360-11010 · 651 bp

Back to Stylosanthes

Pi 0.0466

MarkerSeek score 79.90

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 49
pi: 0.0466
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GTTCGAGTCCGTATAGCCCT	TCATAGTCGTAAAGCAAAAACA	55.0	31.8	58.7	54.4	570-695 bp	1.0	50.1
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	TCATAGTCGTAAAGCAAAAACA	50.0	31.8	57.8	54.4	582-707 bp	1.0	50.0
trnV-UAC-ndhC_p3	GAAGGTCTACGGTTCGAGTC	TCATAGTCGTAAAGCAAAAACA	55.0	31.8	57.5	54.4	581-706 bp	1.0	50.0
trnV-UAC-ndhC_p4	AGAAGGTCTACGGTTCGAGT	TCATAGTCGTAAAGCAAAAACAT	50.0	30.4	57.8	54.9	582-707 bp	1.0	50.0
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	ATCATAGTCGTAAAGCAAAAACA	50.0	30.4	57.8	54.9	583-708 bp	1.0	49.9