Hotspot

ycf1

SSC · 106397-111679 · 5283 bp

Back to Stylidium

Pi 0.0259

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5283
variable_sites: 136
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAATTATTGAACCCGCGCGT	CTTAATACTCTCCGGTGCCG	45.0	55.0	58.3	57.9	256 bp	1.0	79.3
ycf1_p2	TAATTATTGAACCCGCGCGT	TTAATACTCTCCGGTGCCGA	45.0	50.0	58.3	58.2	255 bp	1.0	78.5
ycf1_p3	TAATTATTGAACCCGCGCGT	TGCCGAGATGCTAAAACCAT	45.0	45.0	58.3	57.6	241 bp	1.0	77.2
ycf1_p4	TTAATTATTGAACCCGCGCG	CTTAATACTCTCCGGTGCCG	45.0	55.0	57.3	57.9	257 bp	1.0	76.1
ycf1_p5	TAATTATTGAACCCGCGCGT	GTGCCGAGATGCTAAAACCA	45.0	50.0	58.3	58.6	242 bp	1.0	76.1