Hotspot

rpl32-ndhF

SSC · 121904-122871 · 968 bp

Back to Stylidium

Pi 0.0407

MarkerSeek score 72.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 968
variable_sites: 36
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GGTCGATGGGGAAAAGACTC	ACATAGGAACGGGAAGTGGA	55.0	50.0	58.0	58.1	1392-1463 bp	1.0	86.4
rpl32-ndhF_p2	GTCGATGGGGAAAAGACTCC	ACATAGGAACGGGAAGTGGA	55.0	50.0	58.0	58.1	1391-1462 bp	1.0	86.4
rpl32-ndhF_p3	GTCGATGGGGAAAAGACTCC	AACCAAAATCCCCAACACGA	55.0	45.0	58.0	57.9	1895-1966 bp	1.0	85.7
rpl32-ndhF_p4	GGTCGATGGGGAAAAGACTC	TACATGAAGGGGGAATTGCG	55.0	50.0	58.0	57.9	2075-2146 bp	1.0	85.5
rpl32-ndhF_p5	GTCGATGGGGAAAAGACTCC	TACATGAAGGGGGAATTGCG	55.0	50.0	58.0	57.9	2074-2145 bp	1.0	85.5