Hotspot

rps8-rpl14

LSC · 78623-78805 · 183 bp

Back to Stylidium

Pi 0.071

MarkerSeek score 71.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 13
pi: 0.071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCTATCCAGATCAGCATTTCGT	AATAGTGTCATTGGCTCCCG	40.9	50.0	57.1	57.7	274 bp	1.0	57.6
rps8-rpl14_p2	TCTATCCAGATCAGCATTTCGT	ATAGTGTCATTGGCTCCCGA	40.9	50.0	57.1	58.5	273 bp	1.0	56.2
rps8-rpl14_p3	TCTATCCAGATCAGCATTTCGT	GTCATTGGCTCCCGAAGTAT	40.9	50.0	57.1	57.4	268 bp	1.0	55.3
rps8-rpl14_p4	TTCTATCCAGATCAGCATTTCGT	AATAGTGTCATTGGCTCCCG	39.1	50.0	57.7	57.7	275 bp	1.0	54.9
rps8-rpl14_p5	TCTATCCAGATCAGCATTTCGT	TGTCATTGGCTCCCGAAGTA	40.9	50.0	57.1	58.7	269 bp	1.0	54.5