Hotspot

infA-rps8

LSC · 78125-78217 · 93 bp

Back to Stylidium

Pi 0.0753

MarkerSeek score 72.90

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 93
variable_sites: 7
pi: 0.0753
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GGAAGTGGTTCAGGAATGAAC	CGACTAGAACGAATAGGCGG	47.6	55.0	56.8	58.0	184-193 bp	1.0	65.1
infA-rps8_p2	GGAAGTGGTTCAGGAATGAAC	GCGGTATGGGGGTTGTAATT	47.6	50.0	56.8	57.9	243-252 bp	1.0	64.2
infA-rps8_p3	GGAAGTGGTTCAGGAATGAAC	GAGGGATAATGACAGACCGC	47.6	55.0	56.8	57.8	210-219 bp	1.0	63.8
infA-rps8_p4	GGAAGTGGTTCAGGAATGAAC	TAGAACGAATAGGCGGGGAA	47.6	50.0	56.8	58.2	180-189 bp	1.0	63.7
infA-rps8_p5	GGAAGTGGTTCAGGAATGAAC	GGATAATGACAGACCGCGAG	47.6	55.0	56.8	58.2	207-216 bp	1.0	63.2