Hotspot

petA-psbJ

LSC · 61083-62096 · 1014 bp

Back to Stylidium

Pi 0.0715

MarkerSeek score 82.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 71
pi: 0.0715
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATCCATTACGGGTCCAAGG	CATATTCCGGATTGGGGTCA	55.0	50.0	57.7	57.1	1158-1163 bp	1.0	76.7
petA-psbJ_p2	GATCCATTACGGGTCCAAGG	TGGTTCATATTCCGGATTGGG	55.0	47.6	57.7	57.8	1163-1168 bp	1.0	74.5
petA-psbJ_p3	GATCCATTACGGGTCCAAGG	GTTCATATTCCGGATTGGGGT	55.0	47.6	57.7	57.8	1161-1166 bp	1.0	74.5
petA-psbJ_p4	GATCCATTACGGGTCCAAGG	TATTCCGGATTGGGGTCATC	55.0	50.0	57.7	56.8	1156-1161 bp	1.0	74.4
petA-psbJ_p5	GATCCATTACGGGTCCAAGG	TCATATTCCGGATTGGGGTC	55.0	50.0	57.7	56.8	1159-1164 bp	1.0	74.3