Hotspot

ndhC-trnV-UAC

LSC · 48672-49825 · 1154 bp

Back to Stylidium

Pi 0.0283

MarkerSeek score 73.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1154
variable_sites: 32
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGTCCTTTGATAATCGGG	AGAGCCCAATGCACAAAAGA	55.0	45.0	58.1	58.0	1382-1387 bp	1.0	82.1
ndhC-trnV-UAC_p2	CGGGTCCTTTGATAATCGGG	CGGTTCAAGTCCGTATAGCC	55.0	55.0	58.1	58.2	1287-1292 bp	1.0	81.2
ndhC-trnV-UAC_p3	GGGTCCTTTGATAATCGGGG	AGAGCCCAATGCACAAAAGA	55.0	45.0	57.7	58.0	1381-1386 bp	1.0	80.9
ndhC-trnV-UAC_p4	CGGGTCCTTTGATAATCGGG	GGTTCAAGTCCGTATAGCCC	55.0	55.0	58.1	57.8	1286-1291 bp	1.0	80.5
ndhC-trnV-UAC_p5	CGGGTCCTTTGATAATCGGG	GGTCTTTGAAACAGGTCCGA	55.0	50.0	58.1	57.8	1353-1358 bp	1.0	80.2