Hotspot

trnS-GGA-rps4

LSC · 43764-43943 · 180 bp

Back to Stylidium

Pi 0.0944

MarkerSeek score 80.40

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 17
pi: 0.0944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	ATGAATTGCTGGTGGTAGAA	50.0	40.0	58.1	54.6	261-264 bp	1.0	57.4
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	TGAATTGCTGGTGGTAGAAT	50.0	40.0	58.1	54.6	260-263 bp	1.0	57.4
trnS-GGA-rps4_p3	GTAGGAAAGATGGCCGAGTG	AATGAATTGCTGGTGGTAGA	55.0	40.0	58.1	54.6	312-315 bp	1.0	56.8
trnS-GGA-rps4_p4	GTAGGAAAGATGGCCGAGTG	ATGAATTGCTGGTGGTAGAA	55.0	40.0	58.1	54.6	311-314 bp	1.0	56.8
trnS-GGA-rps4_p5	GTAGGAAAGATGGCCGAGTG	TGAATTGCTGGTGGTAGAAT	55.0	40.0	58.1	54.6	310-313 bp	1.0	56.8