Hotspot

trnK-UUU-rps16

LSC · 3918-4718 · 801 bp

Back to Stylidium

Pi 0.0517
MarkerSeek score 77.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
801
variable_sites
40
pi
0.0517
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAGGCAGGCGTTTTTAAGGA 55.0 45.0 58.0 58.0 967-982 bp 1.0 85.7
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG AGGCAGGCGTTTTTAAGGAA 55.0 45.0 58.0 58.0 966-981 bp 1.0 85.7
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG AAGGCAGGCGTTTTTAAGGA 55.0 45.0 58.0 58.0 966-981 bp 1.0 85.7
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG AGGCAGGCGTTTTTAAGGAA 55.0 45.0 58.0 58.0 965-980 bp 1.0 85.7
trnK-UUU-rps16_p5 TCCCTTTCAGGATCAGTCGT AGGCAGGCGTTTTTAAGGAA 50.0 45.0 58.1 58.0 967-982 bp 1.0 85.3