Hotspot

rps15-ycf1

IRa · 127666-128042 · 377 bp

Back to Strumaria

Pi 0.0936

MarkerSeek score 73.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: IRa
length_bp: 377
variable_sites: 75
pi: 0.0936
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACAAATAATCCAGCAGCCGT	ATCTCCCAGACGTCGTAGAG	45.0	55.0	57.9	58.0	782-895 bp	0.857143	84.9
rps15-ycf1_p2	CCACCGTGTTACATAAGGCA	ATCTCCCAGACGTCGTAGAG	50.0	55.0	57.9	58.0	1189-1303 bp	0.857143	83.7
rps15-ycf1_p3	TTTTTCGTAGACCCCCTTGC	ATCTCCCAGACGTCGTAGAG	50.0	55.0	58.1	58.0	844 bp	0.142857	44.6
rps15-ycf1_p4	TTTTTCGTAGACCCCCTTGC	ATGGAATGCCCCCTTGAATC	50.0	50.0	58.1	57.9	1255 bp	0.142857	44.2
rps15-ycf1_p5	TTTTTCGTAGACCCCCTTGC	GATGGAATGCCCCCTTGAAT	50.0	50.0	58.1	57.9	1256 bp	0.142857	44.2