Hotspot

psaC-ndhE

SSC · 121339-121860 · 522 bp

Back to Strumaria

Pi 0.0423

MarkerSeek score 72.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 522
variable_sites: 43
pi: 0.0423
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	GGAAGCAGCTATTGGGCTAG	50.0	55.0	58.0	58.1	699-789 bp	0.857143	82.3
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	GGGTCACTTCACTGGTTTGT	50.0	50.0	58.0	57.9	1330-1415 bp	0.857143	81.7
psaC-ndhE_p3	GGACAAGCGGATTCACATCT	GTTGGAGATGGGGTCACTTC	50.0	55.0	58.0	57.9	1340-1425 bp	0.857143	81.2
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GGGTCACTTCACTGGTTTGT	50.0	50.0	58.1	57.9	1256-1341 bp	0.857143	81.1
psaC-ndhE_p5	TTCTCGAGTACGGGCTTTTG	GGGTCACTTCACTGGTTTGT	50.0	50.0	57.9	57.9	1552 bp	0.142857	44.7