Hotspot

rpl32-trnL-UAG

SSC · 117401-118108 · 708 bp

Back to Strumaria

Pi 0.0301
MarkerSeek score 69.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
708
variable_sites
50
pi
0.0301
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TAGACACGGCACTATGAGCT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1189-1212 bp 0.714286 71.5
rpl32-trnL-UAG_p2 TAGACACGGCACTATGAGCT TGGCACTTGGCACAGTTATT 50.0 45.0 58.2 58.0 1626-1650 bp 0.714286 71.3
rpl32-trnL-UAG_p3 TAGACACGGCACTATGAGCT TGGCGAAACCTTGGGTAAAA 50.0 45.0 58.2 57.9 1647-1671 bp 0.714286 70.7
rpl32-trnL-UAG_p4 TAGACACGGCACTATGAGCT GGGTAAAAATGGCACTTGGC 50.0 50.0 58.2 57.9 1635-1659 bp 0.714286 70.5
rpl32-trnL-UAG_p5 TAGACACGGCACTATGAGCT ACTGAAAATAACGACCCGCA 50.0 45.0 58.2 57.8 1780-1804 bp 0.714286 69.5