Hotspot

petD-rpoA

LSC · 79193-79396 · 204 bp

Back to Strumaria

Pi 0.0232

MarkerSeek score 65.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 14
pi: 0.0232
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCGCGAAATACCACGATGTA	ACTCTACAGAAGCATTTCGCA	50.0	42.9	58.2	57.7	252-287 bp	1.0	76.3
petD-rpoA_p2	GGTACCGCAGTAGCTCTTTG	ACTCTACAGAAGCATTTCGCA	55.0	42.9	58.4	57.7	340-375 bp	1.0	73.7
petD-rpoA_p3	GTACCGCAGTAGCTCTTTGG	ACTCTACAGAAGCATTTCGCA	55.0	42.9	58.4	57.7	339-374 bp	1.0	73.7
petD-rpoA_p4	CCGCGAAATACCACGATGTA	CACTCTACAGAAGCATTTCGC	50.0	47.6	58.2	57.4	253-288 bp	1.0	73.7
petD-rpoA_p5	CCGCAGTAGCTCTTTGGTTA	ACTCTACAGAAGCATTTCGCA	50.0	42.9	57.6	57.7	336-371 bp	1.0	73.6