Hotspot

rpl32-trnL-UAG

SSC · 114740-115656 · 917 bp

Back to Strophanthus

Pi 0.022

MarkerSeek score 64.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 917
variable_sites: 30
pi: 0.022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGCAACAAACAAAAACAAA	TTAGAAGATGACATGCCGCC	30.0	50.0	52.5	58.0	1037 bp	0.333333	13.3
rpl32-trnL-UAG_p2	GTGCAACAAACAAAAACAAA	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	52.5	57.9	983 bp	0.333333	13.3
rpl32-trnL-UAG_p3	GTGCAACAAACAAAAACAAA	AGCAGCGTGTCTACCAATTT	30.0	45.0	52.5	57.8	976 bp	0.333333	13.3
rpl32-trnL-UAG_p4	GTGCAACAAACAAAAACAAA	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	52.5	57.8	982 bp	0.333333	13.3
rpl32-trnL-UAG_p5	GTGCAACAAACAAAAACAAA	CTAAGAGCAGCGTGTCTACC	30.0	55.0	52.5	57.8	981 bp	0.333333	13.3