Hotspot

ndhF-rpl32

SSC · 113765-114571 · 807 bp

Back to Strophanthus

Pi 0.0134

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 807
variable_sites: 16
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGAAAAAGAAGAAGCCCC	CGCTGTCCAATATCCCTTCC	50.0	55.0	58.1	58.1	978-1007 bp	1.0	80.9
ndhF-rpl32_p2	GGTATGAGCCACCCGTATTG	CGCTGTCCAATATCCCTTCC	55.0	55.0	58.1	58.1	922-951 bp	1.0	80.8
ndhF-rpl32_p3	ATAGGAACGGGAAGTGGGAT	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	946-975 bp	1.0	80.2
ndhF-rpl32_p4	GAACGGGAAGTGGGATGAAA	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	942-971 bp	1.0	80.2
ndhF-rpl32_p5	AGGTATGAGCCACCCGTATT	CGCTGTCCAATATCCCTTCC	50.0	55.0	58.2	58.1	923-952 bp	1.0	80.2