Hotspot

trnP-UGG-psaJ

LSC · 68031-68406 · 376 bp

Back to Strophanthus

Pi 0.0523

MarkerSeek score 77.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 21
pi: 0.0523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CTCGCATTGAAAACCCTCCT	50.0	50.0	58.1	58.2	449-474 bp	1.0	85.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CTCGCATTGAAAACCCTCCT	50.0	50.0	58.1	58.2	448-473 bp	1.0	85.4
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	TCTCGCATTGAAAACCCTCC	50.0	50.0	58.1	58.2	450-475 bp	1.0	85.4
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	TCTCGCATTGAAAACCCTCC	50.0	50.0	58.1	58.2	449-474 bp	1.0	85.4
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	CTCGCATTGAAAACCCTCCT	50.0	50.0	58.1	58.2	460-485 bp	1.0	85.3