Hotspot

psaI-ycf4

LSC · 61115-61556 · 442 bp

Back to Strophanthus

Pi 0.0128

MarkerSeek score 65.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 8
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	574-586 bp	1.0	81.2
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	577-589 bp	1.0	81.2
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	584-596 bp	1.0	80.7
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	568-580 bp	1.0	80.7
psaI-ycf4_p5	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	57.9	57.9	587-599 bp	1.0	80.6