Hotspot

ndhC-trnV-UAC

LSC · 51741-52881 · 1141 bp

Back to Strophanthus

Pi 0.0171
MarkerSeek score 73.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1141
variable_sites
28
pi
0.0171
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGTTAATCGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1286-1584 bp 1.0 81.5
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1272-1570 bp 1.0 81.5
ndhC-trnV-UAC_p3 GTCCTTTGTTAATCGGGGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1285-1583 bp 1.0 78.8
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1271-1569 bp 1.0 78.7
ndhC-trnV-UAC_p5 TGGTCCTTTGTTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1288-1586 bp 1.0 78.5