Hotspot

trnF-GAA-ndhJ

LSC · 49384-50074 · 691 bp

Back to Strophanthus

Pi 0.0131

MarkerSeek score 64.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 13
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	805-833 bp	1.0	83.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	769-797 bp	1.0	83.2
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	840-868 bp	1.0	82.4
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	793-821 bp	1.0	82.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	804-832 bp	1.0	82.2