Hotspot

ycf3-trnS-GGA

LSC · 45707-46227 · 521 bp

Back to Strophanthus

Pi 0.0141

MarkerSeek score 64.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 11
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTCCCTTTCTCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	57.9	58.1	683-706 bp	1.0	80.5
ycf3-trnS-GGA_p2	CCCTTTCTCCCGAAGTTGTC	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.2	58.1	681-704 bp	1.0	79.8
ycf3-trnS-GGA_p3	CTCCCTTTCTCCCGAAGTTG	TTCCAATGCTACGCCTTGAA	55.0	45.0	57.9	57.8	654-677 bp	1.0	79.8
ycf3-trnS-GGA_p4	CCCTTTCTCCCGAAGTTGTC	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.2	57.8	652-675 bp	1.0	79.1
ycf3-trnS-GGA_p5	CTCCCTTTCTCCCGAAGTTG	ACCCTCGGTAAACAAAAGCC	55.0	50.0	57.9	58.4	684-707 bp	1.0	78.2