Hotspot

psbM-trnD-GUC

LSC · 30595-31469 · 875 bp

Back to Strophanthus

Pi 0.0162

MarkerSeek score 62.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 21
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	991-1000 bp	1.0	70.4
psbM-trnD-GUC_p2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	995-1004 bp	1.0	69.8
psbM-trnD-GUC_p3	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.2	58.5	998-1007 bp	1.0	69.1
psbM-trnD-GUC_p4	AGTAGAAAGGCGGTAGGAAC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	998-1007 bp	1.0	67.8
psbM-trnD-GUC_p5	GTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	997-1006 bp	1.0	67.8