Hotspot

rpoB-trnC-GCA

LSC · 26971-28238 · 1268 bp

Back to Strophanthus

Pi 0.0179
MarkerSeek score 74.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1268
variable_sites
33
pi
0.0179
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGCCAAACCCTGATCAATGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.5 58.1 1401-1426 bp 1.0 79.9
rpoB-trnC-GCA_p2 CGCCAAACCCTGATCAATGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.5 58.4 1383-1408 bp 1.0 77.7
rpoB-trnC-GCA_p3 AAAGTTCTTCCGCCAAACCC CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 1411-1436 bp 1.0 76.4
rpoB-trnC-GCA_p4 CTCATTTCCATCCCCGAGC CCGGATTTGAACTGGGGAAA 57.9 50.0 58.0 58.1 1326-1351 bp 1.0 76.3
rpoB-trnC-GCA_p5 ATTCCCTCATTTCCATCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1331-1356 bp 1.0 75.8