Hotspot

trnK-UUU-rps16

LSC · 4247-5010 · 764 bp

Back to Strophanthus

Pi 0.0198
MarkerSeek score 66.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
764
variable_sites
22
pi
0.0198
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCAAAGGGTGGGGGTTTTT 55.0 45.0 57.9 57.7 884-915 bp 1.0 82.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTTCAAAGGGTGGGGGTTTT 55.0 45.0 57.9 57.7 885-916 bp 1.0 82.3
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC TTTTCAAAGGGTGGGGGTTT 55.0 45.0 57.9 57.7 886-917 bp 1.0 82.2
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TTTTTCAAAGGGTGGGGGTT 55.0 45.0 57.9 57.7 887-918 bp 1.0 82.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCAAAGGGTGGGGGTTTTT 50.0 45.0 58.2 57.7 822-853 bp 1.0 81.9