Hotspot

matK-trnK-UUU

LSC · 3513-4209 · 697 bp

Back to Strophanthus

Pi 0.0177

MarkerSeek score 61.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 18
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ATGGTCATGAGCAAGTGCAT	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.9	58.2	824-827 bp	1.0	79.5
matK-trnK-UUU_p2	ATGGTCATGAGCAAGTGCAT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.9	58.2	823-826 bp	1.0	79.5
matK-trnK-UUU_p3	ATGGTCATGAGCAAGTGCAT	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.9	57.5	837-840 bp	1.0	77.1
matK-trnK-UUU_p4	ATGGTCATGAGCAAGTGCAT	TTCGGGTTGCTAACTCAACG	45.0	50.0	57.9	58.5	840-843 bp	1.0	76.8
matK-trnK-UUU_p5	TGGTCATGAGCAAGTGCATA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.2	58.2	822-825 bp	1.0	74.3