Hotspot

ndhA

SSC · 126704-129229 · 2526 bp

Back to Strombocarpa

Pi 0.00475

MarkerSeek score 48.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2526
variable_sites: 18
pi: 0.00475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.3	57.6	2674-2747 bp	1.0	57.3
ndhA_p2	AACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	40.9	50.0	57.0	57.6	2675-2748 bp	1.0	55.2
ndhA_p3	TGAACCCAGTTACCATAAGGA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.0	57.6	2677-2750 bp	1.0	55.0
ndhA_p4	GAACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2676-2749 bp	1.0	54.6
ndhA_p5	TGAACCCAGTTACCATAAGGAAC	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2677-2750 bp	1.0	54.6