Hotspot

accD-psaI

LSC · 64488-65052 · 565 bp

Back to Strombocarpa

Pi 0.0224

MarkerSeek score 54.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 19
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	AGAGGGTAAGTTGCTGAGAGT	45.0	47.6	58.0	58.1	681-699 bp	1.0	77.4
accD-psaI_p2	TTGATTCAATCGTACCGCGT	GGGTAAGTTGCTGAGAGTTGT	45.0	47.6	58.0	57.9	678-696 bp	1.0	77.2
accD-psaI_p3	TCAATCGTACCGCGTAATCC	AGAGGGTAAGTTGCTGAGAGT	50.0	47.6	57.9	58.1	676-694 bp	1.0	76.4
accD-psaI_p4	CAATCGTACCGCGTAATCCT	AGAGGGTAAGTTGCTGAGAGT	50.0	47.6	57.9	58.1	675-693 bp	1.0	76.4
accD-psaI_p5	TCAATCGTACCGCGTAATCC	GGGTAAGTTGCTGAGAGTTGT	50.0	47.6	57.9	57.9	673-691 bp	1.0	76.2