Hotspot

trnM-CAU-atpE

LSC · 57672-57936 · 265 bp

Back to Strombocarpa

Pi 0.0226
MarkerSeek score 57.00
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
265
variable_sites
9
pi
0.0226
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 358-364 bp 1.0 79.8
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG CGAGCTAGGACACGAGTAGA 50.0 55.0 58.4 58.1 350-356 bp 1.0 79.6
trnM-CAU-atpE_p3 TATTGCTTTCATACGGCGGG TCTCAGACGAGCTAGGACAC 50.0 55.0 58.4 58.3 357-363 bp 1.0 78.0
trnM-CAU-atpE_p4 GTATTGCTTTCATACGGCGG CTCTCAGACGAGCTAGGACA 50.0 55.0 57.3 58.0 359-365 bp 1.0 77.7
trnM-CAU-atpE_p5 GTATTGCTTTCATACGGCGG CGAGCTAGGACACGAGTAGA 50.0 55.0 57.3 58.1 351-357 bp 1.0 77.6