Hotspot

trnT-UGU-trnL-UAA

LSC · 50990-52239 · 1250 bp

Back to Strombocarpa

Pi 0.008

MarkerSeek score 51.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1250
variable_sites: 15
pi: 0.008
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1303-1332 bp	1.0	82.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1311-1340 bp	1.0	82.4
trnT-UGU-trnL-UAA_p3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.4	58.0	1275-1304 bp	1.0	82.0
trnT-UGU-trnL-UAA_p4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.4	58.1	1283-1312 bp	1.0	81.5
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1317-1346 bp	1.0	81.1