Hotspot

psbZ-trnG-UCC

LSC · 40524-41121 · 598 bp

Back to Strombocarpa

Pi 0.0167

MarkerSeek score 62.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 15
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	711-753 bp	1.0	82.9
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	714-756 bp	1.0	81.4
psbZ-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	707-749 bp	1.0	81.3
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	712-754 bp	1.0	81.0
psbZ-trnG-UCC_p5	CTTCTCCTGATGGTTGGTCG	ATACATAAATAGGGGCGCGG	55.0	50.0	58.0	57.6	796 bp	0.666667	63.4