Hotspot

petN-psbM

LSC · 31929-33127 · 1199 bp

Back to Strombocarpa

Pi 0.00945

MarkerSeek score 55.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1199
variable_sites: 17
pi: 0.00945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1342-1361 bp	1.0	70.7
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1281-1300 bp	1.0	69.3
petN-psbM_p3	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1291-1310 bp	1.0	69.0
petN-psbM_p4	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1292-1311 bp	1.0	69.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1342-1361 bp	1.0	66.5