Hotspot

rpl32-trnL-UAG

SSC · 112382-112885 · 504 bp

Back to Strobilanthes

Pi 0.039

MarkerSeek score 78.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 504
variable_sites: 54
pi: 0.039
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGTTTTTGTGCGACAAACA	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	55.2	57.9	497-576 bp	1.0	68.2
rpl32-trnL-UAG_p2	AGTTTTTGTGCGACAAACAA	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	55.2	57.9	496-575 bp	1.0	68.2
rpl32-trnL-UAG_p3	AAGTTTTTGTGCGACAAACA	AGCAGCGTGTCTACCAATTT	35.0	45.0	55.2	57.8	490-569 bp	1.0	67.8
rpl32-trnL-UAG_p4	AGTTTTTGTGCGACAAACAA	AGCAGCGTGTCTACCAATTT	35.0	45.0	55.2	57.8	489-568 bp	1.0	67.8
rpl32-trnL-UAG_p5	AGTTTTTGTGCGACAAACAA	CTAAGAGCAGCGTGTCTACC	35.0	55.0	55.2	57.8	494-573 bp	1.0	67.7