Hotspot

ycf2-psbA

LSC · 91980-92103 · 124 bp

Back to Strobilanthes

Pi 0.0671

MarkerSeek score 72.30

Primer available yes

Label ycf2-psbA

Feature Metadata

label_name: ycf2-psbA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 23
pi: 0.0671
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-psbA_p1	CCGGGTTATTTCGGGGTAAG	GGAACCATGCATAGCACTGA	55.0	50.0	58.0	58.0	849-914 bp	1.0	88.1
ycf2-psbA_p2	ATTGGTCCGGGTTATTTCGG	GGAACCATGCATAGCACTGA	50.0	50.0	58.0	58.0	855-920 bp	1.0	88.1
ycf2-psbA_p3	CCGGGTTATTTCGGGGTAAG	AGGAACCATGCATAGCACTG	55.0	50.0	58.0	58.0	850-915 bp	1.0	88.1
ycf2-psbA_p4	TGAAGAAGGGGAGAGGGAAG	GGAACCATGCATAGCACTGA	55.0	50.0	58.0	58.0	1001-1066 bp	1.0	88.0
ycf2-psbA_p5	TGAAGAAGGGGAGAGGGAAG	AGGAACCATGCATAGCACTG	55.0	50.0	58.0	58.0	1002-1067 bp	1.0	87.9