Hotspot

rpl33-rps18

LSC · 67004-67200 · 197 bp

Back to Strobilanthes

Pi 0.0293

MarkerSeek score 68.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 19
pi: 0.0293
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TGAGACCCTGTTCTTCGAGT	CGGTCGATTCAGCTCTTTCA	50.0	50.0	58.0	58.0	727-747 bp	0.888889	79.4
rpl33-rps18_p2	ACTGAGACCCTGTTCTTCGA	ATAAGATCGGGCTTCTCGGA	50.0	50.0	58.0	58.0	858-878 bp	0.888889	79.0
rpl33-rps18_p3	ACTGAGACCCTGTTCTTCGA	TAAGATCGGGCTTCTCGGAT	50.0	50.0	58.0	58.0	857-877 bp	0.888889	79.0
rpl33-rps18_p4	TGAGACCCTGTTCTTCGAGT	ATAAGATCGGGCTTCTCGGA	50.0	50.0	58.0	58.0	856-876 bp	0.888889	79.0
rpl33-rps18_p5	TGAGACCCTGTTCTTCGAGT	TAAGATCGGGCTTCTCGGAT	50.0	50.0	58.0	58.0	855-875 bp	0.888889	79.0