Hotspot

trnP-TGG-psaJ

LSC · 66032-66326 · 295 bp

Back to Strobilanthes

Pi 0.0454

MarkerSeek score 79.20

Primer available yes

Label trnP-TGG-psaJ

Feature Metadata

label_name: trnP-TGG-psaJ
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 38
pi: 0.0454
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-TGG-psaJ_p1	CCCGTGACATTTTGTACCCA	CCGAACCAGAGCGTACTTAG	50.0	55.0	58.1	57.8	382-432 bp	1.0	89.3
trnP-TGG-psaJ_p2	ACCCGTGACATTTTGTACCC	CCGAACCAGAGCGTACTTAG	50.0	55.0	58.1	57.8	383-433 bp	1.0	89.2
trnP-TGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CCGAACCAGAGCGTACTTAG	50.0	55.0	58.1	57.8	394-444 bp	1.0	89.0
trnP-TGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CCGAACCAGAGCGTACTTAG	50.0	55.0	58.1	57.8	393-443 bp	1.0	89.0
trnP-TGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CCGAACCAGAGCGTACTTAG	50.0	55.0	58.1	57.8	396-446 bp	1.0	88.9