Hotspot

psaA-ycf3

LSC · 41225-41904 · 680 bp

Back to Strobilanthes

Pi 0.0187

MarkerSeek score 67.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 37
pi: 0.0187
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	AGCCCTTACCCCAGGTAATT	50.0	50.0	58.5	58.0	773-867 bp	1.0	83.1
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	58.5	58.0	823-917 bp	1.0	82.8
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	58.5	58.0	822-916 bp	1.0	82.8
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	804-898 bp	1.0	82.6
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	733-827 bp	1.0	82.2