Hotspot

trnE-UUC-trnT-GGU

LSC · 30179-30902 · 724 bp

Back to Strobilanthes

Pi 0.0467

MarkerSeek score 82.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 75
pi: 0.0467
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	489-832 bp	1.0	90.4
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	526-869 bp	1.0	90.1
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	468-811 bp	1.0	90.1
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	505-848 bp	1.0	89.9
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	502-845 bp	1.0	89.7